![N-连接和简单O-连接聚糖的ProZyme/酶解糖基化试剂盒[GK80110]/GK80110/1试剂盒](https://www.ebiomall.cn/images/prozyme/201712/GK80110_large.jpg?v=1465602050)
Description
(formerlyGE50)
Containsallenzymes&reagentsneededtoremoveallN-linkedglycans&simpleO-linkedglycans(includingpolysialylated)fromglycoproteins.Seetheproductmanualforanexampleusingbovinefetuin.
N-Glycanase(PNGaseF)cleavesallN-linkedcomplex,hybridorhighmannoseoligosaccharidesunlessα(1,3)corefucosylated(asparaginemustbepeptide-bondedatbothtermini).
Unfortunatelythereisnosuch“universal”endoglycosidaseforO-glycans,thesehavetobetrimmeddowntoGal-GalNAc-O-S/TbeforeO-glycanase(GK80090)canremovetheGal-GalNAcstructure.Modificationofthiscorewithe.g.sialicacidwillblockO-glycanase.
SialidaseA(GK80040)isincludedinthekitandcan removeterminalsialicacidfromsialylatedTantigen/Core1(Sia-Gal-GalNAc-O-S/T)torevealcore1Gal-GalNAc.The GK80115extenderkit(β(1,4)Galactosidase&β-N-Acetylglucosaminidase),availableseparately,canassist withremovalofe.g.core2structures(GlcNAc-Gal-GalNAc-S/T).However,formoreextended&complexO-glycansstructurestheseenzymeswillnotbesufficienttorevealGal-GalNAc.FormoreonO-GalNAcglycancorestructures,pleaseseereference[1].
Size:1kit(~2mgofglycoprotein)
ProductCode:GK80110
1. BrockhausenI,SchachterH,andStanleyP. EssentialsofGlycoBIOLOGy.2ndedition. ColdSpringHarbor(NY):ColdSpringHarborLaboratoryPress;2009.Chapter29, O-GalNAcGlycans.
