Description

Releasesα(2-3,6)-linkedsialicacidfromoligosaccharides,glycoproteins,complexcarbohydrates.

Source:
RecombinantfromClostridiumperfringens,expressedinE.coli.FormerlyknownasproductcodeGE24. 

Specificity:
Thisenzymereleasesα2-3,andα2-6linkedN-acetylneuraminicacidfromcomplexcarbohydrates.ThisenzymewillnotefficientlycleaveNeuAcα2-6linkedtoN-acetylgalactosamine(GalNAc)whentheGalNAcislabeledwithafluorophore.ForthisapplicationSialidaseA(GK80040)isrecommended. 

Figure1:CleavagespecificityforGK80030SialidaseC.

Sialidasesarealsoknownasneuraminidases.

Shipswith(retailpack):
WS00495xReactionBufferB[250mMsodiumphosphatepH6.0]

ReactionBuffer:
5Xconcentratedbufferwhichwhendilutedgives50mMsodiumphosphatepH6.0.

Formulation:
20mMTrisHClpH7.5,containing25mMNaCl

pHoptimum/range:
6.0

UnitDefinition:
Oneunitisdefinedastheamountofenzymerequiredtocatalyzethereleaseof1μmoleofp-nitrophenolfromp-nitrophenyl-α-D-N-acetylneuraminicacidperminuteat37°C,pH5.5.

Size:1U(100µl)

Concentration: ≥10U/ml

ProductCode: GK80030