Description

CleaveswithintheN,N"-diacetylchitobiose(GlcNAc-GlcNAc)coreofhighmannoseandsomehybridtypeN-glycans. EndoglycosidaseH,mannosyl-glycoproteinendo-beta-N-acetylglucosaminidase. 

Source:
RecombinantgenefromStreptomycesplicatus¹,expressedinE.coli. 

Specificity:
CleavesbetweentheGlcNAcresiduesofthechitobiose(GlcNAc-GlcNAc) coreofN-linkedglycans.Oligomannose&somehybridtypeN-glycansarereleasedfromglycoproteins,leavingasingleGlcNAcattachedtoasparagine.ThisspecificityisdistinctfromEndoF2(GKE-5008)whichalsocleavesbiantennarycomplextypeN-glycans,andPNGaseF(GKE-5006)whichreleasesmostN-glycansbycleaving betweentheinnermostGlcNAcandasparagine.

Figure1:CleavagespecificityforGKE-5002EndoH.

R₁:Man_2-150
R₂: H,monosaccharideoroligosaccharideatC2and/orC4
R₃: GlcNAc±Fuc,AsnorDol-pp

Applications:
SelectivereleaseofhighmannoseN-linkedoligosaccharidesfromglycopeptides/glycoproteins. Theenzymehasfoundextensiveuseinthecharacterizationofglycoproteins,dolichol-linkedglycansandthebiosyntheticpathwayforN-glycosylation.

Shipswith: 

• WS00795xReactionBufferforGKE-5002(500mMSodiumCitrate/Phosphate,pH5.5) 
• WS0012DenaturationSolution(2%SDS/1MβME)
• WS0013DetergentSolution(15%DetergentSolution)

Formulation:
50mMsodiumphosphatepH7.5,25mMEDTA, and0.05%sodiumazide

pHoptimum:
5.5

UnitDefinition:
OneunitisdefinedastheamountofenzymerequiredtocatalyzethereleaseofN-linkedoligosaccharidesfrom60μmolesofdenaturedribonucleaseBin1hourat37°C,pH5.5.

Size:30mU(200µl)

Concentration: ≥0.15U/ml

ProductCode: GKE-5002

1.RobbinsPW,TrimbleRB,WirthDF,HeringC,MaleyF,MaleyGF,DasR,GibsonBW,RoyalN,BiemannK. PrimarystructureoftheStreptomycesenzymeendo-beta-N-acetylglucosaminidaseH. JBiolChem.1984Jun25;259(12):7577-83.