![ProZyme/Glyko®PNGase F（天然），100亩[GKE-5003]/GKE-5003/100亩](https://www.ebiomall.cn/images/prozyme/201712/GKE-5003_large.jpg?v=1439315085)
Description
NativePNGaseF,peptide-N-glycosidaseF,peptide-N4-(N-acetyl-β-glucosaminyl)asparagineamidase. ReleasesintactN-glycansbycleaving betweentheinnermostGlcNAcandAsn. Alsoavailableasarecombinantproduct,seeGKE-5006A.
Source:
Native,purifiedfrom Elizabethkingiameningosepticum.Thesourceorganismwaspreviouslyknownas Chryseobacterium[Flavobacterium]meningosepticum[1].
Specificity:
PNGaseF releasesintactN-linkedoligosaccharidesfromglycoproteinsandglycopeptides.Theenzymewillnotcleaveoligosaccharidesfromasingleasparagineresidueandcleavagedoesnotoccurifthereiscoreα1-3linkedfucoseascommonlyencounteredinplantglycoproteinsPhosphate,sulfateandsialicacidgroupsattachedtotheoligosaccharidedonotaffectcleavage.
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Figure1:CleavagespecificityforGKE-5003N-Glycanase(PNGaseF).
R1:N&CsubstitutionotherthanH
R2: maybeHortherestofanoligosaccharide
R3: maybeHor α(1-6)-linkedfucose
Applications:
- releaseofintactN-linkedglycansfromglycopeptidesandglycoproteins
- structure-functionstudiesofN-glycosylatedglycoproteins
- preparationofdeglycosylatedproteinsformolecularweightestimationorcrystallographystudies
Shipswith:
- 1eaWS00675xReaction Buffer
- 1eaWS0012DenaturationSolution
- 1eaWS0013DetergentSolution
Formulation:
52mMTris-HCl,36mMNaCl,9mMsodiumacetate,5mMEDTA,0.02%sodiumazide(pH7.5),glycerol-freeformulation
pHoptimum:
7.5
UnitDefinition:
OneunitofN-GlycanaseisdefinedastheamountofenzymerequiredtocatalyzethereleaseofN-linkedoligosaccharidesfrom1μmoleofdenaturedribonucleaseBperminuteatpH7.5and37̊C.
Size:100mU(50µl)
Concentration: ≥2U/ml
ProductCode:GKE-5003
1.KimKK,KimMK,LimJH,ParkHY,LeeST. TransferofChryseobacteriummeningosepticumandChryseobacteriummiricolatoElizabethkingiagen.nov.asElizabethkingiameningosepticacomb.nov.andElizabethkingiamiricolacomb.nov. IntJSystEvolMicrobiol.2005May;55(Pt3):1287-93.
