Description

Releasesnon-reducingterminalα(1-2,3,6)-linkedmannosefromoligosaccharides. Theenzymeexhibitssomekineticpreferenceforα(1-2,3)-linkedmannoseoverα(1-6)-linkedmannose. 

Source:
Jackbean.

Specificity:
Theenzymehasbroadspecificity,cleavingα(1-2,3,6)-linkedmannose,althoughsomekineticpreferencehasbeenobserved(α1-2,6>3).Theenzymewillnotcleaveasingleα(1-6)linkedmannoseresiduefromcoreβ-mannose,butwill,however,removeasingleα1-3linkedmannosefromthecoreβ-mannose. Byusingenzymeconcentrationsofaround50U/mlandextendedincubationtimes(upto18hours)at37°C,completeremovalofallnon-reducingterminalαlinkedmannoseresiduesmaybeachieved.

Toexpediteglycansequencingstudies,thesluggishactivityofGKX-5010JackBeanαmannosidasetowardα(1-6)-linkedmannoseresiduescanbeovercomebyusingtheenzymeincombinationwiththeαmannosidasefromXanthomonasmannihotis whichrapidlycleavesα(1-6)linkages.TheX.mannihotisα-mannosidase mayinhibittheactionofothermannosidasesifabranched(α1-6)mannoseispresentinthesubstrate,soistypicallyaddedafterincubationofthesubstratewithJackbeanα-mannosidase(GKX-5010).

Figure1:CleavagespecificityforGKX-5010 α(1-2,3,6)-Mannosidase.

Shipswith:
WS0095GKX-5010IncubationBuffer(500mMsodiumacetate,10mMzincchloride(pH5.0).

ReactionBuffer:
5Xconcentratedbufferwhichwhendilutedgives100mMsodiumacetatepH5.0containing2mMZn2+.

Formulation:
20mMTris-HCl,20mMNaCl(pH7.5)

MW:~190kD

pHoptimum/range:
4.5

UnitDefinition:
Oneunitisdefinedastheamountofenzymethatwillhydrolyze1μmoleofpNP-α-mannopyranosideperminuteatpH4.5and37°C.

Size:10U(70μl)

Concentration: ≥150U/ml

ProductCode:GKX-5010