Description
Releasesunsubstituted,Ser/Thr-linkedGalGalNAcfromproteins;usedwithadditionalenzymestoremovemorecomplexO-linkedstructures.Alsoknownas Endo-O-Glycosidase™,Endo-αN-acetylgalactosaminidase,O-Glycosidase.
Source:
RecombinantgenefromStreptococcuspneumoniae,expressedinE.coli.
Specificity:
Theenzymeishighlyspecific,liberatingGalβ1-3GalNAcfromserineorthreonineresiduesaloneoraspartofglycopeptidesorglycoproteins.Thereisnoapparentpreferenceforserineoverthreonine.Substitutionofthedisaccharidecorewithsialicacid,fucose,N-acetylglucosamineorN-acetylgalactosamineresidues,preventscleavageoftheglycosylaminelinkage.AbsenceofthegalactoseresidueortheacetamidogroupontheinnermostN-acetylgalactosamineresiduealsopreventscleavageoftheglycosylaminelinkage.
O-Glycanaseisincludedinour EnzymaticDeglycosylationKitforN-Linked&SimpleO-LinkedGlycans(GK80110),whichmaybeusedinconjunctionwithour prO-LINKExtender™KitforComplexO-LinkedGlycans(GK80115).
Applications:
- DetectionofO-glycosylationinproteins
- Epitopeandbindingsiteanalysis
- BioactivityinO-glycans
- Analysisofbiosynthesisofglycoproteins
- StructuralanalysisofO-glycan
- Glycoproteindeglycosylation
Shipswith:
WS00595XreactionbufferA[250mMsodiumphosphatepH5.0]
Formulation:
20mMTrisHClpH7.5containing25mMNaCl.
ReactionBuffer:
5Xconcentratedbufferwhichwhendilutedgives50mMsodiumphosphatepH5.0.
MW:
~180kD
pHoptimum/range:
5.5/5.0-7.0
UnitDefinition:
Oneunitisdefinedastheamountofenzymerequiredtocatalyzethereleaseof1μmoleofGalβ1-3GalNAcα1-p-nitrophenolperminuteat37°C,pH5.5.
Size:50mU(40µl)
Concentration: ≥1.25U/ml
ProductCode:GK80090