Description

PNGaseF,peptide-N-glycosidaseF,peptide-N⁴-(N-acetyl-β-glucosaminyl)asparagineamidase. ReleasesintactN-glycansbycleaving betweentheinnermostGlcNAcandAsn. SamemanufacturingprocessasN-GlycanaseforGlykoPrep(EDTA-free),suppliedatthe GKE-5006 concentrationof ≥2.5U/ml.

Source:
RecombinantgenefromElizabethkingiameningosepticum,expressedinE.coli.Thesourceorganismwaspreviouslyknownas Chryseobacterium[Flavobacterium]meningosepticum[1]. 

Specificity: 
CleavesallN-linkedcomplex,hybridorhighmannoseoligosaccharides,unlessα(1-3)corefucosylated,asinplantglycans.Asparaginemustbepeptide-bondedatbothtermini.Phosphate,sulfateandsialicacidgroupsattachedtotheoligosaccharidedonotaffectcleavage.EndoFfree.

Figure1:CleavagespecificityforGKE-5016N-Glycanase(PNGaseF).
R₁:N&CsubstitutionotherthanH
R₂: maybeHortherestofanoligosaccharide
R₃: maybeHor α(1-6)-linkedfucose

"A&B"packsizesshipwith:

• 1eaWS00105xN-GlycanaseReactionBuffer
• 1eaWS0012DenaturationSolution
• 1eaWS0013DetergentSolution
• 1eaWS01455xN-GlycanaseTrisReactionBuffer(MSapplications)

"D"packsizedoesnotshipwiththeabovebuffers. Buffersareavailablewith"D"packsizeonrequest.

Formulation:
20mMTris-HCl,50mMNaCl(pH7.5)

ReactionBuffer:
5Xconcentratedbuffer,whendilutedgives20mMsodiumphosphatepH7.5,containing0.02%sodiumazide.

MW:
~35,000Daltons

pHoptimum/range:
8.6/7.5-9.5

UnitDefinition:
OneunitisdefinedastheamountofenzymerequiredtocatalyzethereleaseofN-linkedoligosaccharidesfrom1μmoleofdenaturedribonucleaseBperminuteat37°C,pH7.5.

NotformulatedforFACE®analysis,useGK50050instead.

Size:1Uin400µl

Concentration: ≥2.5U/ml

ProductCode:GKE-5016D

1.KimKK,KimMK,LimJH,ParkHY,LeeST. TransferofChryseobacteriummeningosepticumandChryseobacteriummiricolatoElizabethkingiagen.nov.asElizabethkingiameningosepticacomb.nov.andElizabethkingiamiricolacomb.nov. IntJSystEvolMicrobiol.2005May;55(Pt3):1287-93.